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Effect of α-ALA on <t>AMPKα1</t> in HDF +STZ induced diabetic mice. ( A ) Relative mRNA expression of AMPKα1; ( B ) Western blot analyses of AMPKα1 and p-AMPKα1; ( C ) AMPKα1 protein quantifications; ( D ) p-AMPKα1 protein quantifications. Control: Normal control mice; DHD: HDF +STZ; α-ALA-L: HDF +STZ mice treated with 2 g/kg of α-ALA; α-ALA-H: HDF +STZ mice treated with 4 g/kg of α-ALA. Data are expressed as mean ± SD, n ≥5. # P < 0.05, ## P < 0.01 is compared with the Control group. *P < 0.05, **P < 0.01 is compared with the DHD group.
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Effect of α-ALA on <t>AMPKα1</t> in HDF +STZ induced diabetic mice. ( A ) Relative mRNA expression of AMPKα1; ( B ) Western blot analyses of AMPKα1 and p-AMPKα1; ( C ) AMPKα1 protein quantifications; ( D ) p-AMPKα1 protein quantifications. Control: Normal control mice; DHD: HDF +STZ; α-ALA-L: HDF +STZ mice treated with 2 g/kg of α-ALA; α-ALA-H: HDF +STZ mice treated with 4 g/kg of α-ALA. Data are expressed as mean ± SD, n ≥5. # P < 0.05, ## P < 0.01 is compared with the Control group. *P < 0.05, **P < 0.01 is compared with the DHD group.
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Effect of α-ALA on <t>AMPKα1</t> in HDF +STZ induced diabetic mice. ( A ) Relative mRNA expression of AMPKα1; ( B ) Western blot analyses of AMPKα1 and p-AMPKα1; ( C ) AMPKα1 protein quantifications; ( D ) p-AMPKα1 protein quantifications. Control: Normal control mice; DHD: HDF +STZ; α-ALA-L: HDF +STZ mice treated with 2 g/kg of α-ALA; α-ALA-H: HDF +STZ mice treated with 4 g/kg of α-ALA. Data are expressed as mean ± SD, n ≥5. # P < 0.05, ## P < 0.01 is compared with the Control group. *P < 0.05, **P < 0.01 is compared with the DHD group.
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Effect of α-ALA on AMPKα1 in HDF +STZ induced diabetic mice. ( A ) Relative mRNA expression of AMPKα1; ( B ) Western blot analyses of AMPKα1 and p-AMPKα1; ( C ) AMPKα1 protein quantifications; ( D ) p-AMPKα1 protein quantifications. Control: Normal control mice; DHD: HDF +STZ; α-ALA-L: HDF +STZ mice treated with 2 g/kg of α-ALA; α-ALA-H: HDF +STZ mice treated with 4 g/kg of α-ALA. Data are expressed as mean ± SD, n ≥5. # P < 0.05, ## P < 0.01 is compared with the Control group. *P < 0.05, **P < 0.01 is compared with the DHD group.

Journal: Diabetes, Metabolic Syndrome and Obesity

Article Title: α-ALA Attenuates Cardiac Injury in Diabetic Heart Disease Mice by Modulating Inflammation and AMPKα1

doi: 10.2147/DMSO.S537517

Figure Lengend Snippet: Effect of α-ALA on AMPKα1 in HDF +STZ induced diabetic mice. ( A ) Relative mRNA expression of AMPKα1; ( B ) Western blot analyses of AMPKα1 and p-AMPKα1; ( C ) AMPKα1 protein quantifications; ( D ) p-AMPKα1 protein quantifications. Control: Normal control mice; DHD: HDF +STZ; α-ALA-L: HDF +STZ mice treated with 2 g/kg of α-ALA; α-ALA-H: HDF +STZ mice treated with 4 g/kg of α-ALA. Data are expressed as mean ± SD, n ≥5. # P < 0.05, ## P < 0.01 is compared with the Control group. *P < 0.05, **P < 0.01 is compared with the DHD group.

Article Snippet: The membranes were incubated overnight with primary antibodies specific for AMPKα1 (1:2000, 10,929-2-AP, Proteintech, China), p-AMPKα1 (1:500, AF3423, Affinity, China), and β-actin (1:10,000, 81,115-1-RR, Proteintech, China), followed by horseradish peroxidase-conjugated secondary antibodies.

Techniques: Expressing, Western Blot, Control